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KMID : 0368420040470010042
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Journal of Plant Biology
2004 Volume.47 No. 1 p.42 ~ p.47
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Analysis of carbon metabolism in transgenicArabidopsis thaliana transformed with the cyanobacterial sucrose phosphate synthase gene
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Yoon Seok-Yoon
Lee Jang-Wook
Bhoo Seong-Hee
Jeon Jong-Seong
Lee Youn-Hyung
Hahn Tae-Ryong
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Abstract
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To investigate the effects of sucrose-phosphate synthase (SPS) on carbon partitioning, transgenicArabidopsis plants transformed withSynechocystis SPS were constructed. The integration, copy number and expression level were confirmed by Southern and Northern blot analyses. SPS activity in leaves from the transgenic and wild type plants was not significantly different. The level of sucrose and starch in the leaves of transgenic plant were slightly decreased compared to wild type. The glucose and fructose contents were increased up to two-fold compared to wild type during the light period. It is our speculation that the decreased sucrose level of the transgenic plant might be caused by the high acid invertase.
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KEYWORD
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sucrose phosphate synthase, carbon metabolism, Arabidopsis, carbon metabolite, SPS activity
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